Induced neuronal differentiation of human embryonic stem cells.

  title={Induced neuronal differentiation of human embryonic stem cells.},
  author={Maya Schuldiner and Rachel Eiges and Amir Eden and Ofra Yanuka and Joseph Itskovitz-Eldor and Ronald S Goldstein and Nissim Benvenisty},
  journal={Brain research},
  volume={913 2},
Human embryonic stem (ES) cells are pluripotent cells capable of forming differentiated embryoid bodies (EBs) in culture. We examined the ability of growth factors under controlled conditions to increase the number of human ES cell-derived neurons. Retinoic acid (RA) and nerve growth factor (betaNGF) were found to be potent enhancers of neuronal differentiation, eliciting extensive outgrowth of processes and the expression of neuron-specific molecules. Our findings show that human ES cells have… CONTINUE READING