Induced differentiation of a transformed clone of Ly-1+ B cells by clonal T cells and antigen.

@article{Bishop1986InducedDO,
  title={Induced differentiation of a transformed clone of Ly-1+ B cells by clonal T cells and antigen.},
  author={Gail A Bishop and Geoffrey Haughton},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1986},
  volume={83 19},
  pages={7410-4}
}
The present study used cocultures of clonally derived B and T cells, together with an antigen reactive with the membrane Ig of the clonal B cells, to address the issue of B-cell differentiation requirements. The B cells were CH12.LX, an in vitro grown subclone of a murine B-cell lymphoma, which bears surface IgM reactive with sheep erythrocytes. The T cells were alloreactive T-helper-cell hybridomas. Very small numbers of T-helper cells could induce differentiation of cloned B cells without the… CONTINUE READING