Induced allostery in the directed evolution of an enantioselective Baeyer-Villiger monooxygenase.

@article{Wu2010InducedAI,
  title={Induced allostery in the directed evolution of an enantioselective Baeyer-Villiger monooxygenase.},
  author={Sheng Gan Wu and Juan Pablo Acevedo and Manfred T Reetz},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2010},
  volume={107 7},
  pages={2775-80}
}
The molecular basis of allosteric effects, known to be caused by an effector docking to an enzyme at a site distal from the binding pocket, has been studied recently by applying directed evolution. Here, we utilize laboratory evolution in a different way, namely to induce allostery by introducing appropriate distal mutations that cause domain movements with concomitant reshaping of the binding pocket in the absence of an effector. To test this concept, the thermostable Baeyer-Villiger… CONTINUE READING
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