Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

  title={Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells},
  author={Junying Yu and Maxim A. Vodyanik and Kim Smuga-Otto and Jessica Antosiewicz-Bourget and Jennifer L. Frane and Shulan Tian and Jeff Nie and Gudrun A. Jonsdottir and Victor Ruotti and Ron M. Stewart and Igor I. Slukvin and James A. Thomson},
  pages={1917 - 1920}
Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. [] Key Result These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful…

Monitoring Multiple Pluripotency Biomarkers After Delivery of Reprogramming Factors to Human Somatic Cells

This investigation demonstrates that expression of these four factors, plus the additional transcription factor Nanog, from recombinant lentivirus, effectively reprogrammed human somatic cells such that they displayed a pluripotent phenotype and expressed multiple pluriopotency genes.

Generation of human-induced pluripotent stem cells

This protocol describes how to establish primary human fibroblasts lines and how to derive iPS cells by retroviral transduction of reprogramming factors, and takes 2 months to complete reprograming human primary fibro Blasts starting from biopsy.

Generation of induced pluripotent stem cells from human blood.

The ability to reprogram cells from human blood will allow the generation of patient-specific stem cells for diseases in which the disease-causing somatic mutations are restricted to cells of the hematopoietic lineage.

Reprogramming of human somatic cells to pluripotency with defined factors

The data demonstrate that defined factors can reprogramme human cells to pluripotency, and establish a method whereby patient-specific cells might be established in culture.

Induced pluripotent stem cells: emerging techniques for nuclear reprogramming.

Current techniques of generating iPS cells and mechanisms of nuclear reprogramming are reviewed, and the potential for therapeutic applications is discussed.

Late Passage Human Fibroblasts Induced to Pluripotency Are Capable of Directed Neuronal Differentiation

Successful reprogramming of human fibroblasts after more than 20 passages in vitro, to a pluripotent state with four transcription factors: Oct4, Sox2, Klf4, and c-Myc is reported.

Induced Pluripotent Stem Cells Generated Without Viral Integration

This work generated mouse induced pluripotent stem cells from fibroblasts and liver cells by using nonintegrating adenoviruses transiently expressing Oct4, Sox2, Klf4, and c-Myc, providing strong evidence that insertional mutagenesis is not required for in vitro reprogramming.

Mouse induced pluripotent stem cells.

The recent discovery that it is possible to directly reprogramme somatic cells to an embryonic stem (ES) cell-like pluripotent state, by retroviral transduction of just four genes represents a major breakthrough in stem cell research.



Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells

It is demonstrated that reprogrammed pluripotent cells can be isolated from genetically unmodified somatic donor cells solely based upon morphological criteria.

In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state

The results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells.

Embryonic stem cell lines derived from human blastocysts.

Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate

Generation of germline-competent induced pluripotent stem cells

iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application.

Overexpression of NANOG in human ES cells enables feeder-free growth while inducing primitive ectoderm features

It is shown that NANOG overexpression in HESCs enables their propagation for multiple passages during which the cells remain pluripotent, significant in the understanding of self-renewal and pluripotency pathways in H ESCs, and of their use for modeling early development in humans.

Nanog promotes transfer of pluripotency after cell fusion

It is reported that in fusions between ES cells and neural stem (NS) cells, increased levels of Nanog stimulate pluripotent gene activation from the somatic cell genome and enable an up to 200-fold increase in the recovery of hybrid colonies, all of which show ES cell characteristics.

Human Embryonic Stem Cells Reprogram Myeloid Precursors Following Cell–Cell Fusion

The results indicate that hESCs are capable of reprogramming nuclei from differentiated cells and that the hESC hybrid cells provide a new model system for studying the mechanisms of nuclear reprograming.

Apoptosis and differentiation of human embryonic stem cells induced by sustained activation of c-Myc

Activation of c-MycER in hES cells induced apoptosis and differentiation into extraembryonic endoderm and trophectoderm lineages concomitant with reduced expression of the pluripotent markers Oct4 and Nanog, suggesting an important role for the integrin/extracellular matrix interaction in the regulation of ES cell behavior.