Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

  title={Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells},
  author={Junying Yu and Maxim A. Vodyanik and Kim Smuga-Otto and Jessica Antosiewicz-Bourget and Jennifer L. Frane and Shulan Tian and Jeff Nie and Gudrun A. Jonsdottir and Victor Ruotti and Ron M. Stewart and Igor I. Slukvin and James A. Thomson},
  pages={1917 - 1920}
Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. [] Key Result These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful…

Monitoring Multiple Pluripotency Biomarkers After Delivery of Reprogramming Factors to Human Somatic Cells

This investigation demonstrates that expression of these four factors, plus the additional transcription factor Nanog, from recombinant lentivirus, effectively reprogrammed human somatic cells such that they displayed a pluripotent phenotype and expressed multiple pluriopotency genes.

Generation of human-induced pluripotent stem cells

This protocol describes how to establish primary human fibroblasts lines and how to derive iPS cells by retroviral transduction of reprogramming factors, and takes 2 months to complete reprograming human primary fibro Blasts starting from biopsy.

Generation of induced pluripotent stem cells from human blood.

The ability to reprogram cells from human blood will allow the generation of patient-specific stem cells for diseases in which the disease-causing somatic mutations are restricted to cells of the hematopoietic lineage.

Reprogramming of human somatic cells to pluripotency with defined factors

The data demonstrate that defined factors can reprogramme human cells to pluripotency, and establish a method whereby patient-specific cells might be established in culture.

Late Passage Human Fibroblasts Induced to Pluripotency Are Capable of Directed Neuronal Differentiation

Successful reprogramming of human fibroblasts after more than 20 passages in vitro, to a pluripotent state with four transcription factors: Oct4, Sox2, Klf4, and c-Myc is reported.

Induced Pluripotent Stem Cells Generated Without Viral Integration

This work generated mouse induced pluripotent stem cells from fibroblasts and liver cells by using nonintegrating adenoviruses transiently expressing Oct4, Sox2, Klf4, and c-Myc, providing strong evidence that insertional mutagenesis is not required for in vitro reprogramming.

Mouse induced pluripotent stem cells.

The recent discovery that it is possible to directly reprogramme somatic cells to an embryonic stem (ES) cell-like pluripotent state, by retroviral transduction of just four genes represents a major breakthrough in stem cell research.

Induced pluripotent stem (iPS) cells from human fetal stem cells.

  • P. Guillot
  • Biology
    Best practice & research. Clinical obstetrics & gynaecology
  • 2016

Pluripotent stem cell lines.

The derivation of human embryonic stem cells 10 years ago ignited an explosion of public interest in stem cells, yet this achievement depended on prior decades of research on mouse embryonic



Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells

It is demonstrated that reprogrammed pluripotent cells can be isolated from genetically unmodified somatic donor cells solely based upon morphological criteria.

In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state

The results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells.

Embryonic stem cell lines derived from human blastocysts.

Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate

Nanog promotes transfer of pluripotency after cell fusion

It is reported that in fusions between ES cells and neural stem (NS) cells, increased levels of Nanog stimulate pluripotent gene activation from the somatic cell genome and enable an up to 200-fold increase in the recovery of hybrid colonies, all of which show ES cell characteristics.

Apoptosis and differentiation of human embryonic stem cells induced by sustained activation of c-Myc

Activation of c-MycER in hES cells induced apoptosis and differentiation into extraembryonic endoderm and trophectoderm lineages concomitant with reduced expression of the pluripotent markers Oct4 and Nanog, suggesting an important role for the integrin/extracellular matrix interaction in the regulation of ES cell behavior.

Human embryonic stem cell-derived CD34+ cells: efficient production in the coculture with OP9 stromal cells and analysis of lymphohematopoietic potential.

Data indicate that CD34+ cells generated through hES/OP9 coculture display several features of definitive hematopoietic stem cells.


Recently, many cellular oncogenes were identified in human cancerous cells by the DNA transfection method, some of which were found to contain sequences related with already known viral oncogenees.