Induced Pluripotent Stem (iPS) Cells

  title={Induced Pluripotent Stem (iPS) Cells},
  author={Kursad Turksen and Andras Nagy},
  booktitle={Methods in Molecular Biology},
Somatic cell reprogramming to induced pluripotent stem cells (iPSCs) is a revolutionary technology, with repercussions affecting modern functional genomics and regenerative medicine. Still, relatively little is known about the processes underlying this dramatic cellular and molecular metamorphosis. Reprogramming technology based on the implementation of piggyBac (PB) transposons has enabled studies of iPSC reprogramming mechanisms, shedding an increasing light on these processes. Unique… 
2 Citations

Organoids at the PUB: The Porcine Urinary Bladder Serves as a Pancreatic Niche for Advanced Cancer Modeling

The establishment of the porcine urinary bladder (PUB) is revealed as an advanced organ culture model for shaping an ex vivo pancreatic niche and advances the existing pancreatic cancer models by adding feasibility, complexity, and customization at low cost and high flexibility.



Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon

P piggyBac transposon–based reprogramming may be used to generate therapeutically applicable iPSCs and could be identified by negative selection.

Divergent reprogramming routes lead to alternative stem-cell states

It is proved that the pluripotent spectrum can encompass multiple, unique cell states, and by maintaining elevated reprogramming factor expression levels, mouse embryonic fibroblasts go through unique epigenetic modifications to arrive at a stable, Nanog-positive, alternative pluripotency state.

Reprogramming in vivo produces teratomas and iPS cells with totipotency features

It is shown that transitory induction of the four factors Oct4, Sox2, Klf4 and c-Myc in mice results in teratomas emerging from multiple organs, implying that full reprogramming can occur in vivo.

Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2

Valproic acid (VPA), a histone deacetylase inhibitor, enables reprogramming of primary human fibroblasts with only two factors, Oct4 and Sox2, without the need for the oncogenes c-Myc or Klf4, and supports the possibility of reprograming through purely chemical means.

piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells

It is shown that the individual PB insertions can be removed from established iPS cell lines, providing an invaluable tool for discovery, and the traceless removal of reprogramming factors joined with viral 2A sequences delivered by a single transposon from murine iPS lines is demonstrated.

Transgene-free production of pluripotent stem cells using piggyBac transposons.

PiggyBac transposition provides an effective means to generate human, transgene-free iPSCs, delivering reprogramming factors via plasmid transfection and piggyBAC transposition.

Virus free induction of pluripotency and subsequent excision of reprogramming factors

It is shown that non-viral transfection of a single multiprotein expression vector, which comprises the coding sequences of c-Myc, Klf4, Oct4 and Sox2 linked with 2A peptides, can reprogram both mouse and human fibroblasts and can be removed once reprogramming has been achieved.