Indoxyl sulfate induces epithelial-to-mesenchymal transition in rat kidneys and human proximal tubular cells.

@article{Bolati2011IndoxylSI,
  title={Indoxyl sulfate induces epithelial-to-mesenchymal transition in rat kidneys and human proximal tubular cells.},
  author={Dilinaer Bolati and Hidehisa Shimizu and Yukihiro Higashiyama and Fuyuhiko Nishijima and Toshimitsu Niwa},
  journal={American journal of nephrology},
  year={2011},
  volume={34 4},
  pages={318-23}
}
BACKGROUND/AIMS Indoxyl sulfate (IS) is a uremic toxin that accelerates the progression of chronic kidney disease (CKD). This study aimed to determine if IS induces epithelial-to-mesenchymal transition (EMT) in the kidneys of hypertensive rats and human proximal tubular cells (HK-2). METHODS EMT was evaluated by immunohistochemistry, reverse transcription-polymerase chain reaction and immunoblotting of the epithelial markers E-cadherin and zonula occludens-1 (ZO-1), and the mesenchymal marker… CONTINUE READING