Individually distinct tumor-specific cell surface antigen identified by monoclonal antibody on a Rous sarcoma virus-induced mouse tumor.

@article{Kuzumaki1982IndividuallyDT,
  title={Individually distinct tumor-specific cell surface antigen identified by monoclonal antibody on a Rous sarcoma virus-induced mouse tumor.},
  author={Noboru Kuzumaki and Hidehiko Minakawa and Toru Miyazaki and Shuka Haraguchi and Tsuyoshi Matsuo and Takato O. Yoshida},
  journal={Journal of the National Cancer Institute},
  year={1982},
  volume={69 2},
  pages={527-30}
}
Hybrid cell lines were prepared by the fusion of BALB/c myeloma P3U-1 cells with the lymphocytes of BALB/c mice that were immunized with syngeneic Rous sarcoma virus (RSV)-induced tumor CSA1M cells. Three clones of the hybrid progeny (3.4B2, 3.4C6, and 3.5C11) produced cytotoxic IgM antibodies against CSA1M cells. One of the clones, 3.5C11, was chosen for analysis of the detailed specificity. Both direct cytotoxicity assays and absorption tests revealed that monoclonal antibody from 3.5C11 was… CONTINUE READING