Individual-specific ‘fingerprints’ of human DNA

@article{Jeffreys1985IndividualspecificO,
  title={Individual-specific ‘fingerprints’ of human DNA},
  author={A. Jeffreys and V. Wilson and S. Thein},
  journal={Nature},
  year={1985},
  volume={316},
  pages={76-79}
}
Simple tandem-repetitive regions of DNA (or ‘minisatellites’) which are dispersed in the human genome frequently show substantial length polymorphism arising from unequal exchanges which alter the number of short tandem repeats in a minisatellite1–4. We have shown previously that the repeat elements in a subset of human minisatellites share a common 10–15-base-pair (bp) ‘core’ sequence which might act as a recombination signal in the generation of these hypervariable regions5. A hybridization… Expand
Positive identification of an immigration test-case using human DNA fingerprints
TLDR
The potential for positive or inclusive identification is illustrated and the DNA fingerprint analysis of an immigration case is described, the resolution of which would have been very difficult and laborious using currently available single-locus genetic markers. Expand
Characterization of a panel of highly variable minisatellites cloned from human DNA
TLDR
Five of the most variable loci detected in human DNA by hybridization with DNA fingerprint probes have been cloned and characterized, and are illustrated by an analysis of forensic specimens from two victims who had been sexually assaulted and murdered. Expand
Characterization of a VNTR sequence on human Chromosome 22 revealing polymorphic cross-hybridizing sequences in mammals
Hypervariable regions, also termed "minisatellites" or variable number of tandem repeats (VNTR), first discovered in the human genome (Wyman and White 1980), consist of a core tandem repeat sequenceExpand
A GGCAGG motif in minisatellites affecting their germline instability.
TLDR
The results indicate that the loci containing mo-1 homologues with mutation in the GGCAGG sequence are monomorphic despite the other mutants showing polymorphism, and strongly suggest that the motif contributes to the germline instability of minisatellites. Expand
DNA "fingerprints" and segregation analysis of multiple markers in human pedigrees.
TLDR
Tandem-repetitive DNA hybridization probes based on a putative human recombination signal detect multiple polymorphic minisatellite fragments in human DNA and can be applied to linkage analysis in human pedigrees. Expand
Simultaneous genetic mapping of multiple human minisatellite sequences using DNA fingerprinting.
TLDR
The results of these analyses suggest that the distribution of minisatellites in the human genome is skewed toward telomeres and is highly clustered in character. Expand
Genome scanning by two‐dimensional DNA typing: The use of repetitive DNA sequences for rapid mapping of genetic traits
TLDR
An electrophoretic system, two‐dimensional DNA typing, which may fulfill the requirements of fully exploring the various sets of mammalian repeat sequences and can greatly accellerate the mapping of genetic traits in humans, animals, and plants. Expand
Forensic Use of Short Tandem Repeats via PCR
TLDR
The DNA sequence of man’s chromosomes has the potential for wide variation from individual to individual since only 1% encodes functional elements, i.e. genes, and the large number of alleles provides a very powerful DNA-based personal identification method. Expand
Detection of recombination in variable number tandem repeat sequences
TLDR
The goal is to develop an adequate tool for the detection of recombinant among a set of minisatellite sequences by combining a multiple alignment tool and a method based on phylogenetic profiling, and a first solution, called MS PhylPro, is designed. Expand
Characterisation of minisatellite-associated meiotic recombination hotspots in the human genome
TLDR
Analysis of the flanking DNA was extended to the MS31A locus to reveal significant levels of linkage disequilibrium suggesting low frequency of recombination in these regions, indicating that both minisatellites mutate by the same mechanism. Expand
...
1
2
3
4
5
...

References

SHOWING 1-9 OF 9 REFERENCES
Hypervariable ‘minisatellite’ regions in human DNA
TLDR
A probe based on a tandem-repeat of the core sequence can detect many highly variable loci simultaneously and can provide an individual-specific DNA ‘fingerprint’ of general use in human genetic analysis. Expand
Molecular basis of length polymorphism in the human zeta-globin gene complex.
TLDR
The homology between the three length polymorphisms may be an indication of the presence of a more widespread group of related sequences in the human genome, which might be useful for generalized linkage studies. Expand
DNA sequence variants in the G γ-, A γ-, δ- and β-globin genes of man
TLDR
Three types of variant restriction enzyme patterns of globin DNA fragments were detected in otherwise normal individuals and can be used to derive an approximate estimate of the total number of different DNA sequence variants in man. Expand
The structure of the human zeta-globin gene and a closely linked, nearly identical pseudogene
TLDR
Comparison of the human zeta- and alpha-globin gene sequences reveals that the embryonic and adult alpha-like genes began to diverge from each other relatively early in vertebrate evolution. Expand
The highly polymorphic region near the human insulin gene is composed of simple tandemly repeating sequences
The sequence of the highly polymorphic region near the human insulin gene, which begins 363 base pairs before the start of transcription and extends upstream, indicated that it is generated byExpand
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
A series of plasmid vectors containing the multiple cloning site (MCS7) of M13mp7 has been constructed. In one of these vectors a kanamycin-resistance marker has been inserted into the center of theExpand
Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue
DNA sequence analysis of the activated oncogene from the T24 human bladder carcinoma line and two alleles of its normal cellular progenitor (c-Ha-ras-1) indicates that the genes encompass at leastExpand
Gel electrophoresis of restriction fragments.
TLDR
Gel electrophoresis of restriction fragments of deoxyribonucleic acid (DNA) in agarose or polyacrylamide gels is discussed, which is often useful to identify a particular sequence in the DNA fragments separated by gel electrophoreis. Expand
The establishment of lymphoblastoid lines from adult and fetal human lymphoid tissue and its dependence on EBV
Continuous human lymphoblastoid cell lines (LL), derived from lymphoid tissue or peripheral blood of 20 adults without histories of recent infectious mononucleosis at a frequency close to 100 %, wereExpand