Individual-specific ‘fingerprints’ of human DNA

  title={Individual-specific ‘fingerprints’ of human DNA},
  author={A. Jeffreys and V. Wilson and S. Thein},
Simple tandem-repetitive regions of DNA (or ‘minisatellites’) which are dispersed in the human genome frequently show substantial length polymorphism arising from unequal exchanges which alter the number of short tandem repeats in a minisatellite1–4. We have shown previously that the repeat elements in a subset of human minisatellites share a common 10–15-base-pair (bp) ‘core’ sequence which might act as a recombination signal in the generation of these hypervariable regions5. A hybridization… Expand
Positive identification of an immigration test-case using human DNA fingerprints
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Five of the most variable loci detected in human DNA by hybridization with DNA fingerprint probes have been cloned and characterized, and are illustrated by an analysis of forensic specimens from two victims who had been sexually assaulted and murdered. Expand
Characterization of a VNTR sequence on human Chromosome 22 revealing polymorphic cross-hybridizing sequences in mammals
Hypervariable regions, also termed "minisatellites" or variable number of tandem repeats (VNTR), first discovered in the human genome (Wyman and White 1980), consist of a core tandem repeat sequenceExpand
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The results indicate that the loci containing mo-1 homologues with mutation in the GGCAGG sequence are monomorphic despite the other mutants showing polymorphism, and strongly suggest that the motif contributes to the germline instability of minisatellites. Expand
DNA "fingerprints" and segregation analysis of multiple markers in human pedigrees.
Tandem-repetitive DNA hybridization probes based on a putative human recombination signal detect multiple polymorphic minisatellite fragments in human DNA and can be applied to linkage analysis in human pedigrees. Expand
Simultaneous genetic mapping of multiple human minisatellite sequences using DNA fingerprinting.
The results of these analyses suggest that the distribution of minisatellites in the human genome is skewed toward telomeres and is highly clustered in character. Expand
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Forensic Use of Short Tandem Repeats via PCR
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The goal is to develop an adequate tool for the detection of recombinant among a set of minisatellite sequences by combining a multiple alignment tool and a method based on phylogenetic profiling, and a first solution, called MS PhylPro, is designed. Expand
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Analysis of the flanking DNA was extended to the MS31A locus to reveal significant levels of linkage disequilibrium suggesting low frequency of recombination in these regions, indicating that both minisatellites mutate by the same mechanism. Expand


Hypervariable ‘minisatellite’ regions in human DNA
A probe based on a tandem-repeat of the core sequence can detect many highly variable loci simultaneously and can provide an individual-specific DNA ‘fingerprint’ of general use in human genetic analysis. Expand
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The homology between the three length polymorphisms may be an indication of the presence of a more widespread group of related sequences in the human genome, which might be useful for generalized linkage studies. Expand
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Gel electrophoresis of restriction fragments of deoxyribonucleic acid (DNA) in agarose or polyacrylamide gels is discussed, which is often useful to identify a particular sequence in the DNA fragments separated by gel electrophoreis. Expand
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