Individual rotavirus-like particles containing 120 molecules of fluorescent protein are visible in living cells.

@article{Charpilienne2001IndividualRP,
  title={Individual rotavirus-like particles containing 120 molecules of fluorescent protein are visible in living cells.},
  author={Annie Charpilienne and Mohamed Nejmeddine and Mabel Berois and Nathalie Parez and Emmanuelle Neumann and Elizabeth A. Hewat and Germain Trugnan and Jacob Cohen},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 31},
  pages={29361-7}
}
Rotaviruses are large, complex icosahedral particles consisting of three concentric capsid layers. When the innermost capsid protein VP2 is expressed in the baculovirus-insect cell system it assembles as core-like particles. The amino terminus region of VP2 is dispensable for assembly of virus-like particles (VLP). Coexpression of VP2 and VP6 produces double layered VLP. We hypothesized that the amino end of VP2 could be extended without altering the auto assembly properties of VP2. Using the… CONTINUE READING
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