Individual IKs channels at the surface of mammalian cells contain two KCNE1 accessory subunits.

@article{Plant2014IndividualIC,
  title={Individual IKs channels at the surface of mammalian cells contain two KCNE1 accessory subunits.},
  author={Leigh D Plant and Dazhi Xiong and Hui Dai and Steve An Goldstein},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2014},
  volume={111 14},
  pages={E1438-46}
}
KCNE1 (E1) β-subunits assemble with KCNQ1 (Q1) voltage-gated K(+) channel α-subunits to form IKslow (IKs) channels in the heart and ear. The number of E1 subunits in IKs channels has been an issue of ongoing debate. Here, we use single-molecule spectroscopy to demonstrate that surface IKs channels with human subunits contain two E1 and four Q1 subunits. This stoichiometry does not vary. Thus, IKs channels in cells with elevated levels of E1 carry no more than two E1 subunits. Cells with low… CONTINUE READING
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