Indirubin-3'-monoxime, a derivative of a Chinese antileukemia medicine, inhibits P-TEFb function and HIV-1 replication.

@article{Heredia2005Indirubin3monoximeAD,
  title={Indirubin-3'-monoxime, a derivative of a Chinese antileukemia medicine, inhibits P-TEFb function and HIV-1 replication.},
  author={Alonso Heredia and Charles Davis and Douty Bamba and Nhut Le and Muhammad Yalwa Gwarzo and Mariola Sadowska and Robert C. Gallo and Robert R Redfield},
  journal={AIDS},
  year={2005},
  volume={19 18},
  pages={2087-95}
}
OBJECTIVE To evaluate the effects of the cyclin dependent kinase (CDK) inhibitor Indirubin-3'-monoxime (IM) on Tat-mediated transactivation function, a step of the HIV-1 cycle that is not currently targeted in antiviral therapy. METHODS The effects of IM on CDK implicated in HIV-1 Tat transactivation function were evaluated by kinase assays, transfection experiments, RNase protection assay and RT-PCR analysis of viral transcripts. The antiviral effect of IM was investigated in cells from HIV… CONTINUE READING