Indirect somatic embryogenesis in Coffea with different ploidy levels: a revisiting and updating study

  title={Indirect somatic embryogenesis in Coffea with different ploidy levels: a revisiting and updating study},
  author={Nat{\'a}lia Arruda Sanglard and Paulo Marcos Amaral-Silva and Mariana Cansian Sattler and St{\'e}fanie Cristina de Oliveira and Let{\'i}cia Miranda Ces{\'a}rio and Ad{\'e}sio Ferreira and Carlos Roberto Carvalho and Wellington Ronildo Clarindo},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
Indirect somatic embryogenesis (ISE) is required for plant propagation and a prerequisite for applications that may provide new germplasms. Genetic, epigenetic and physiological features of the explant donor are barriers for ISE establishment, hindering its wide use. Despite the identification and/or expression analysis of genes during ISE, no approach to establish the karyotype aspects has been performed so far. So, this study aims to establish the ISE and compare the in vitro responses… 
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