Indirect evidence for two distinct mechanisms of DNA cleavage at the oriT of RP4 during conjugation.

@article{Waterman1993IndirectEF,
  title={Indirect evidence for two distinct mechanisms of DNA cleavage at the oriT of RP4 during conjugation.},
  author={S. R. Waterman and Jim Hackett and Paul A. Manning},
  journal={Plasmid},
  year={1993},
  volume={30 1},
  pages={79-81}
}
Mobilization of a potential Campylobacter shuttle vector from Escherichia coli K-12 into C. hyointestinalis generated a small plasmid harboring a hybrid oriT nick region. This is presumed to have resulted from the cleavage and religation of the oriT of RP4 and the putative nick region of the shuttle vector. These data imply that two distinct DNA cleavage… CONTINUE READING