Indirect enzyme-linked immunosorbent assay for measurement of human immunoglobulins E and G to purified cow's milk proteins: application in diagnosis of cow's milk allergy.

@article{Campbell1987IndirectEI,
  title={Indirect enzyme-linked immunosorbent assay for measurement of human immunoglobulins E and G to purified cow's milk proteins: application in diagnosis of cow's milk allergy.},
  author={Dianne E Campbell and J Ngamphaiboon and Matthew M. Clark and Mary Catherine Harris and G. B. Kolski and Steven D. Douglas},
  journal={Journal of clinical microbiology},
  year={1987},
  volume={25 11},
  pages={2114-9}
}
An indirect double-antibody enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of human immunoglobulin E (IgE) and IgG to the cow's milk proteins (CMP) alpha-casein, alpha-lactalbumin, and beta-lactoglobulin. Human serum albumin was used as the negative-antigen control. Rabbit anti-human IgE or IgG served as the primary antibody… CONTINUE READING