Indirect detection of superoxide in RAW 264.7 macrophage cells using microchip electrophoresis coupled to laser-induced fluorescence.

@article{Campos2015IndirectDO,
  title={Indirect detection of superoxide in RAW 264.7 macrophage cells using microchip electrophoresis coupled to laser-induced fluorescence.},
  author={Richard P S de Campos and Joseph Siegel and Claudia G. Fresta and Giuseppe Caruso and Josx00E9 Alberto Fracassi da Silva and Susan Marie Lunte},
  journal={Analytical and bioanalytical chemistry},
  year={2015},
  volume={407 23},
  pages={7003-12}
}
Superoxide, a naturally produced reactive oxygen species (ROS) in the human body, is involved in many pathological and physiological signaling processes. However, if superoxide formation is left unregulated, overproduction can lead to oxidative damage to important biomolecules, such as DNA, lipids, and proteins. Superoxide can also lead to the formation of peroxynitrite, an extremely hazardous substance, through its reaction with endogenously produced nitric oxide. Despite its importance… CONTINUE READING