Indirect competitive immunoassay for detection of aflatoxin B1 in corn and nut products using the array biosensor.

@article{Sapsford2006IndirectCI,
  title={Indirect competitive immunoassay for detection of aflatoxin B1 in corn and nut products using the array biosensor.},
  author={Kim E. Sapsford and Chris Rowe Taitt and Stephanie Fertig and Martin H. Moore and Michael E. Lassman and Chris M. Maragos and Lisa C. Shriver-Lake},
  journal={Biosensors & bioelectronics},
  year={2006},
  volume={21 12},
  pages={2298-305}
}
Because of the potential health risks of aflatoxin B1 (AFB1), it is essential to monitor the level of this mycotoxin in a variety of foods. An indirect competitive immunoassay has been developed using the NRL array biosensor, offering rapid, sensitive detection and quantification of AFB1 in buffer, corn and nut products. AFB1-spiked foods were extracted with methanol and Cy5-anti-AFB1 added to the resulting sample. The extracted sample/antibody mix was passed over a waveguide surface patterned… CONTINUE READING
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