Indirect competitive ELISA for determination of traces of peanut (Arachis hypogaea L.) protein in complex food matrices.

@article{Holzhauser1999IndirectCE,
  title={Indirect competitive ELISA for determination of traces of peanut (Arachis hypogaea L.) protein in complex food matrices.},
  author={Thomas Holzhauser and Stefan Vieths},
  journal={Journal of agricultural and food chemistry},
  year={1999},
  volume={47 2},
  pages={603-11}
}
An indirect competitive ELISA was developed allowing the detection of hidden peanut protein residues down to 2 ppm (micorgrams per gram) in various foods. The high-titer, peanut-specific polyclonal antiserum used recognized potentially allergenic proteins in both native and roasted peanuts. In the absence of a food matrix, extractable protein from roasted peanuts was detected at 104 +/- 13%. From various food items, peanut protein at > or =13 ppm was recovered between 84 and 126%, and at 2 ppm… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 14 extracted citations

Quantification of bovine β-casein allergen in baked foodstuffs based on ultra-performance liquid chromatography with tandem mass spectrometry

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment • 2015
View 1 Excerpt

Characterization, antigenicity and detection of fish gelatine and isinglass used as processing aids in wines.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment • 2010

Development of a Biological Assay to Determine the Allergenic Potential of Foods

Journal für Verbraucherschutz und Lebensmittelsicherheit • 2006
View 4 Excerpts

Similar Papers

Loading similar papers…