Indirect antigen-trap ELISAs using polyclonal antisera for detection of group B and D Salmonellas in chickens.

Abstract

Indirect antigen-trap ELISAs able to detect Salmonella typhimurium and S. enteritidis are described. Their limits of sensitivity were about 10(7) organisms in nutrient broth culture. With the ELISA developed for S. typhimurium, 76 of 77 strains gave optical density (OD) readings of 1.06 to 1.40. Three other serotypes from group B in the Kaufmann-White… (More)

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