Indigo formation by microorganisms expressing styrene monooxygenase activity.

@article{OConnor1997IndigoFB,
  title={Indigo formation by microorganisms expressing styrene monooxygenase activity.},
  author={Kevin E O'Connor and Alan D W Dobson and Sybe Hartmans},
  journal={Applied and environmental microbiology},
  year={1997},
  volume={63 11},
  pages={4287-91}
}
The transformation of indole to indigo by microorganisms expressing styrene monooxygenase (SMO) has been studied. Styrene and indole are structurally very similar, and thus we looked at a variety of styrene-degrading strains for indole transformation to indigo. Two strains, Pseudomonas putida S12 and CA-3, gave a blue color on solid media when grown in the presence of indole. Indole induces its own transformation on solid media but is a poor inducer in liquid media. Styrene is the best inducer… CONTINUE READING

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