The indigenous knowledge on medicinal plants is gaining recognition worldwide because of its support in discovery of new medicines and its importance for proper conservation of biodiversity. This paper documents the traditional knowledge of medicinal plants used for the treatment of skin diseases by the tribe, namely Kaani, of Kanyakumari District, Tamil Nadu, India. The present study was done through structured questionnaires in consultation with the tribal ethno-medical practitioners and has resulted in the documentation of 55 medicinal plant species belonging to 38 families. For curing the skin disease, the use of aboveground plant parts was higher (83.33%) than the underground plant parts (16.67%). Of the aboveground plant parts, leaves were used in the majority of cases (19 species), followed by whole plants (11 species). Different underground plant forms such as roots and rhizomes were also used by the tribe as medicine. The study thus underlines the potentials of the ethno-botanical research and the need for the documentation of traditional ecological knowledge pertaining to the medicinal plant utilization for the greater benefit of humankind.