Independent regulation of two genes in Escherichia coli by tetracyclines and Tet repressor variants.

@article{Kamionka2004IndependentRO,
  title={Independent regulation of two genes in Escherichia coli by tetracyclines and Tet repressor variants.},
  author={Annette Kamionka and Miriam Sehnal and Oliver Scholz and Wolfgang Hillen},
  journal={Journal of bacteriology},
  year={2004},
  volume={186 13},
  pages={4399-401}
}
We report a regulation system in Escherichia coli for independent regulation of two distinct reporter genes by application of Tet repressors with different specificities. One Tet repressor variant comprises wild-type tet operator (tetO) recognition and exclusive induction with the novel inducer 4-dedimethylamino-anhydrotetracycline. The other Tet repressor variant shows tetO-4C recognition and induction with tetracycline. We demonstrate that both variants are independently active in vivo and… CONTINUE READING