Incremental truncation of PHA synthases results in altered product specificity.


PHA synthase is the key enzyme involved in the biosynthesis of microbial polymers, polyhydroxyalkanoates (PHA). In this study, we created a hybrid library of PHA synthase gene with different crossover points by an incremental truncation method between the C-terminal fragments of the phaC(Cn) (phaC from Cupriavidus necator) and the N-terminal fragments of… (More)
DOI: 10.1016/j.enzmictec.2012.02.003

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