Incremental truncation of PHA synthases results in altered product specificity.

Abstract

PHA synthase is the key enzyme involved in the biosynthesis of microbial polymers, polyhydroxyalkanoates (PHA). In this study, we created a hybrid library of PHA synthase gene with different crossover points by an incremental truncation method between the C-terminal fragments of the phaC(Cn) (phaC from Cupriavidus necator) and the N-terminal fragments of… (More)
DOI: 10.1016/j.enzmictec.2012.02.003

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