Increasing the brightness of cyanine fluorophores for single-molecule and superresolution imaging.


In spite of their relatively low fluorescence quantum yield, cyanine dyes such as Cy3, Cy5, or Cy7 are widely used in single-molecule fluorescence applications due to their high extinction coefficients and excellent photon yields. We show that the fluorescence quantum yield and lifetime of red-emitting cyanine dyes can be substantially increased in heavy… (More)
DOI: 10.1002/cphc.201300874


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