Increasing speed and throughput when using HPLC-MS/MS systems for drug metabolism and pharmacokinetic screening.

  title={Increasing speed and throughput when using HPLC-MS/MS systems for drug metabolism and pharmacokinetic screening.},
  author={Yunsheng Hsieh and Walter A. Korfmacher},
  journal={Current drug metabolism},
  volume={7 5},
Both combinatorial chemistry and parallel synthesis provide a valuable means for the production of large numbers of compounds with diverse molecular architectures that become available for various drug discovery experiments. In both the lead optimization and lead selection stages, one requirement that is common for many processes is the need for bioanalytical support. This review summarizes current high throughput strategies and efficient methodologies that are employed for drug metabolism and… 

HPLC-MS/MS in drug metabolism and pharmacokinetic screening

This review summarizes high performance liquid chromatography coupled to a tandem mass spectrometer (HPLC-MS/MS) methodologies that are employed in drug metabolism and pharmacokinetic screens for a series of new chemical entities.

Potential of HILIC-MS in quantitative bioanalysis of drugs and drug metabolites.

  • Y. Hsieh
  • Biology, Chemistry
    Journal of separation science
  • 2008
This review summarizes the HILIC-MS/MS methods for the trace quantitative determinations of the drug compounds and their metabolites to support both in vitro and in vivo experiments.

Recent advances in high-throughput quantitative bioanalysis by LC-MS/MS.

A Fully Integrated Assay Panel for Early Drug Metabolism and Pharmacokinetics Profiling

An integrated and automated assay panel (DMPK Wave 1) that informs weekly on lipophilicity, solubility, human plasma protein binding, and metabolic stability in rat hepatocytes and human liver microsomes is described.

Recent development in high-throughput bioanalytical support for in vitro ADMET profiling

Recent advances such as automated MS/MS optimization, high-speed and multiplexed LC separation, and integrated software support have significantly increased the speed and quality of ADMET bioanalysis using LC-MS/MS.

An integrated bioanalytical platform for supporting high-throughput serum protein binding screening.

To improve overall efficiency of ADME bioanalysis, an integrated system was proposed featuring an automated and unattended MS/MS optimization, a staggered parallel LC/MS/MS for high-throughput sample analysis, and a sophisticated software tool for liquid chromatography/tandem mass spectrometry raw data review as well as biological data calculation and reporting.

Evaluation of a high-throughput online solid phase extraction-tandem mass spectrometry system for in vivo bioanalytical studies.

Overall, HT-SPE/MS exhibited significant potential for high-throughput in vivo bioanalysis and comparable accuracy, precision, linearity, and sensitivity were achieved with analysis speeds 20-30-fold higher.

The Application of Mass Spectrometry in Drug Metabolism and Pharmacokinetics.

Qualitative and qualitative mass spectrometry approaches and their specific utility in DMPK characterization will be described in this chapter.

Recent advances in metabolite identification and quantitative bioanalysis by LC-Q-TOF MS.

The performance and versatility of LC-Q-TOF MS are thoroughly illustrated by its applications in metabolite identification and quantitative bioanalysis, as well as developments on tandem separation techniques and data acquisition and data-mining methods.