Increasing ecological inference from high throughput sequencing of fungi in the environment through a tagging approach.

@article{Taylor2008IncreasingEI,
  title={Increasing ecological inference from high throughput sequencing of fungi in the environment through a tagging approach.},
  author={D Lee Taylor and Michael G. Booth and Jack W. McFarland and Ian Charold Herriott and Niall J. Lennon and Chad Nusbaum and Thomas G. Marr},
  journal={Molecular ecology resources},
  year={2008},
  volume={8 4},
  pages={742-52}
}
High throughput sequencing methods are widely used in analyses of microbial diversity, but are generally applied to small numbers of samples, which precludes characterization of patterns of microbial diversity across space and time. We have designed a primer-tagging approach that allows pooling and subsequent sorting of numerous samples, which is directed to amplification of a region spanning the nuclear ribosomal internal transcribed spacers and partial large subunit from fungi in… CONTINUE READING

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Identification of indigenous and introduced symbiotic fungi in ectomycorrhizae by amplification of nuclear and mitochondrial ribosomal DNA

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