Increasing Rhynchostylis retusa’s Callus Formation by Immersing Explant in Ascorbic Acid and Planting on Activated Charcoal Contained Medium

@article{Kurniasari2019IncreasingRR,
  title={Increasing Rhynchostylis retusa’s Callus Formation by Immersing Explant in Ascorbic Acid and Planting on Activated Charcoal Contained Medium},
  author={Dwiyana Anela Kurniasari and Astuti and Evy Yulianti and Suyitno and Lili Sugiyarto and Ixora Sartika Mercuriani},
  journal={Journal of Physics: Conference Series},
  year={2019}
}
The aimed of this research is to increase Rhynchostylis retusa callus formation through immersing explants in ascorbic acid and planting it on activated charcoal contained medium. It's an experimental research that consist of three factors; i.e. immersion explants in ascorbic acid (immersed and not immersed), kind of in-vitro culture medium (not-contained (as control), pro-analysis activated charcoal contained and commercial activated charcoal contained), and the type of expants (leaf and root… 
1 Citations
Characterization and Potential of Coelogyne rochussenii Orchids from Bukit Rimbang and Bukit Baling Wildlife Sanctuary as Explant Source
TLDR
The characterization results showed that the young pseudobulbs, young leaves, healthy roots, and physiologically ripe fruits of the C. rochussenii orchids obtained could be used as a source of explants to support ex-situ conservation efforts.

References

SHOWING 1-10 OF 21 REFERENCES
The Response of Node and Leaf Explant of Binahong (Anredera cordifolia L.) on MS Media with Variation of BAP Concentration
The purpose of this study was to determine the response of nodal explants and leaves of plants binahong on MS medium with the addition of 2,4-D with BAP variation. Research was designed using two
Relation between explant age, total phenols and regeneration response in tissue cultured cotton (Gossypium hirsutum L.)
The aim of this study is to determine total phenol amount of the explants and to expose relation between explant age, total phenols and regeneration response for tissue culture and gene transfer
Effects of antioxidants, plant growth regulators and wounding on phenolic compound excretion during micropropagation of Strelitzia reginae
The aim of this study was to determine the effects of antioxidant treatments, plant growth regulators (PGRs) and explants wounding in tissue culture involving Strelitzia reginae on total phenol
THE EFFECT OF SOME ANTIOXIDANTS ON BLACKENING AND GROWTH OF In Vitro CULTURE OF BANANA (Musa spp.cv. GRAND NAINE)
TLDR
Results demonstrated that using activated charcoal (1.5 g/L) in particular, inhibited the blackening phenomena and enhanced the growth and the elongation of banana (Musa spp. cv. Grand Naine).
Control of lethal browning of tissue culture plantlets of Cavendish banana cv. Formosana with ascorbic acid
Cavendish banana cv. Formosana is a high yielding commercial cultivar resistant to race 4 of Fusariumoxysporum f. sp. cubense. Mass micropropagation of this cultivar has a serious problem of high
Induksi Kalus dari Eksplan Daun In Vitro Keladi Tikus (Typhonium sp.) dengan Perlakuan 2,4-D dan Kinetin
TLDR
The results showed that explants could not produce callus, the explants response just a swelling leaf explants, and the treatments that could maintain 66.67% explants growth was the combination 0.4-D + 0.5 mg / L 2.5mg / L kinetin.
ULASAN Masalah Pencoklatan pada Kultur Jaringan
TLDR
The browning of tissue culture and the medium can often be prevented by one of the several different approaches, such as by removing the phenolic compounds produced, modifying the redox potential, inactivating Phenolase enzymes, reducing phenolase activity and substrate availability, as well as pre-treatments by soaking and preconditioning on a basal medium.
Contamination and browning in tissue culture of Platanus occidentalis L.
TLDR
Results indicated that the contamination and browning rates of the material cut off in January and March were somewhat lower than those in July, and the pretreatment of soaking the explants in different anti-oxidants and absorbents at the same time could diminish some side effects.
Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman melalui Kultur Jaringan
TLDR
In plant tissue culture, growth regulator has significant roles such as to control root and shoot development in the plant formation and callus induction and the existence of a certain growth regulating substances can enhance growth regulator activity of other substances.
...
...