Increasing Accuracy and Throughput in Large-Scale Microsatellite Fingerprinting of Cacao Field Germplasm Collections

@article{Motilal2008IncreasingAA,
  title={Increasing Accuracy and Throughput in Large-Scale Microsatellite Fingerprinting of Cacao Field Germplasm Collections},
  author={Lambert A. Motilal and Dapeng Zhang and Pathmanathan Umaharan and Sue Mischke and Michel Boccara and Stephen J. Pinney},
  journal={Tropical Plant Biology},
  year={2008},
  volume={2},
  pages={23-37}
}
In this study we report on increasing the rate and accuracy of microsatellite fingerprinting of accessions in Theobroma cacao L. field germplasm collections with a medium-throughput capillary sequencing system. We examined the use of a reduced number of microsatellite loci to decrease the time and materials required for fingerprinting and determined the best available microsatellite loci for accurately separating accessions. A subset of nine informative loci that could separate sixty accessions… CONTINUE READING

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