Increased yield of homogeneous HIV-1 reverse transcriptase (p66/p51) using a slow purification approach.

@article{Bhikhabhai1993IncreasedYO,
  title={Increased yield of homogeneous HIV-1 reverse transcriptase (p66/p51) using a slow purification approach.},
  author={Rama Bhikhabhai and Tony Carlsson and Torsten Unge and S L{\"o}vgren and Bror Strandberg},
  journal={Journal of chromatography},
  year={1993},
  volume={639 1},
  pages={67-74}
}
A chromatographic procedure to purify recombinant reverse transcriptase (RT) from human immunodeficiency virus-1 is reported. A bacterial system which expressed large amounts of p66 RT polypeptide was used. The purification scheme was optimized for high-yield production of homogeneous p66/p51 RT using a combination of chromatographic matrices in the following order: Q-Sepharose, heparin-Sepharose, phenyl-Sepharose, S-Sepharose, Poly(A)-Sepharose and Q-Sepharose. The p66 polypeptide remained… CONTINUE READING

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