• DOI:10.1152/AJPGI.00114.2007
• Corpus ID: 24133214

Increased visceral sensitivity to capsaicin after DSS-induced colitis in mice: spinal cord c-Fos expression and behavior.

@article{Eijkelkamp2007IncreasedVS,
  title={Increased visceral sensitivity to capsaicin after DSS-induced colitis in mice: spinal cord c-Fos expression and behavior.},
  author={Niels Eijkelkamp and Annemieke Kavelaars and Sigrid Elsenbruch and Manfred Schedlowski and Gerald J. Holtmann and Cobi J. Heijnen},
  journal={American journal of physiology. Gastrointestinal and liver physiology},
  year={2007},
  volume={293 4},
  pages={
          G749-57
        }
}

• N. Eijkelkamp, A. Kavelaars, +3 authors C. Heijnen
• Published 1 October 2007
• Biology, Medicine
• American journal of physiology. Gastrointestinal and liver physiology

During acute and chronic inflammation visceral pain perception is altered. Conflicting data exist, however, on visceral pain perception in the postinflammatory phase. The aim of the present study was to investigate whether visceral pain perception is altered after resolution of dextran sodium sulfate (DSS)-induced inflammation of the colon. Visceral sensory function in mice was assessed by monitoring behavioral responses to intracolonic capsaicin instillation. Two hours later the number of c-Fos-positive neurons in lamina I/II and X of spinal cord segments T(12/13)-S1 was determined as a measure of neuronal activation. DSS colitis was induced by adding 1% of DSS to the drinking water. The course of DSS-induced colitis was assessed by determining the disease activity index score. Animals developed a transient colitis and had recovered at day 49. At this time point, cytokine levels and colon length were similar to control animals. Importantly, after resolution of DSS-induced colitis the behavioral response to intracolonic capsaicin was increased compared with control mice. Moreover, capsaicin-induced spinal cord neuronal c-Fos expression was significantly increased. Interestingly, after colitis animals also exhibited referred somatic hyperalgesia as measured with von Frey hairs on the abdominal wall. We conclude that postinflammatory visceral hyperalgesia occurs after resolution of DSS-induced colitis and that capsaicin-induced behavioral responses and spinal cord neuronal c-Fos activation are effective readouts for determination of visceral pain perception.