Increased uracil insertion in DNA is cytotoxic and increases the frequency of mutation, double strand break formation and VSG switching in Trypanosoma brucei.

@article{CastilloAcosta2012IncreasedUI,
  title={Increased uracil insertion in DNA is cytotoxic and increases the frequency of mutation, double strand break formation and VSG switching in Trypanosoma brucei.},
  author={Victor Manuel Castillo-Acosta and Fernando Aguilar-Pereyra and J. C. J. Bart and Miguel Navarro and Luis Miguel Ru{\'i}z-P{\'e}rez and Antonio E. Vidal and Dolores Gonz{\'a}lez-Pacanowska},
  journal={DNA repair},
  year={2012},
  volume={11 12},
  pages={986-95}
}
Deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) and uracil-DNA glycosylase (UNG) are key enzymes involved in the control of the presence of uracil in DNA. While dUTPase prevents uracil misincorporation by removing dUTP from the deoxynucleotide pool, UNG excises uracil from DNA as a first step of the base excision repair pathway (BER). Here, we report that strong down-regulation of dUTPase in UNG-deficient Trypanosoma brucei cells greatly impairs cell viability in both bloodstream and… CONTINUE READING