Increased transport of pteridines compensates for mutations in the high affinity folate transporter and contributes to methotrexate resistance in the protozoan parasite Leishmania tarentolae.

Abstract

Functional cloning led to the isolation of a novel methotrexate (MTX) resistance gene in the protozoan parasite Leishmania. The gene corresponds to orfG, an open reading frame (ORF) of the LD1/CD1 genomic locus that is frequently amplified in several Leishmania stocks. A functional ORF G-green fluorescence protein fusion was localized to the plasma membrane… (More)

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@article{Kndig1999IncreasedTO, title={Increased transport of pteridines compensates for mutations in the high affinity folate transporter and contributes to methotrexate resistance in the protozoan parasite Leishmania tarentolae.}, author={Christoph K{\"u}ndig and Asmaa Haimeur and D. J. Legare and Blerta Papadopoulou and Marc A. Ouellette}, journal={The EMBO journal}, year={1999}, volume={18 9}, pages={2342-51} }