Increased stability and specificity through combined hybridization of peptide nucleic acid (PNA) and locked nucleic acid (LNA) to supercoiled plasmids for PNA-anchored "Bioplex" formation.

@article{Lundin2005IncreasedSA,
  title={Increased stability and specificity through combined hybridization of peptide nucleic acid (PNA) and locked nucleic acid (LNA) to supercoiled plasmids for PNA-anchored "Bioplex" formation.},
  author={Karin E Lundin and Maroof Hasan and Pedro Md Moreno and Elisabeth T{\"o}rnquist and Iulian I. Oprea and Mathias G. Svahn and E Oscar Simonson and C. I. Edvard Smith},
  journal={Biomolecular engineering},
  year={2005},
  volume={22 5-6},
  pages={185-92}
}
Low cellular uptake and poor nuclear transfer hamper the use of non-viral vectors in gene therapy. Addition of functional entities to plasmids using the Bioplex technology has the potential to improve the efficiency of transfer considerably. We have investigated the possibility of stabilizing sequence-specific binding of peptide nucleic acid (PNA) anchored functional peptides to plasmid DNA by hybridizing PNA and locked nucleic acid (LNA) oligomers as "openers" to partially overlapping sites on… CONTINUE READING