Increased sensitivity for the detection of malignant melanoma cells in peripheral blood using an improved protocol for reverse transcription-polymerase chain reaction.


Conflicting results have been obtained by various research groups using tyrosinase reverse transcription-polymerase chain reaction (RT-PCR) for detecting micrometastases in the blood of melanoma patients, with positive results ranging from 0 to 100% in disseminated melanoma. Methodological differences in the processing of blood samples may in part account… (More)