Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada.

@article{Angelis1992IncreasedRT,
  title={Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada.},
  author={Karel J. Angelis and Jindřich Bř{\'i}za and J Satava and I Sk{\'a}kal and Jiř{\'i} Velem{\'i}nsk{\'y} and Josef Vlas{\'a}k and Karol Kleibl and G. P. Margison},
  journal={Mutation research},
  year={1992},
  volume={273 3},
  pages={271-80}
}
The protein coding region of the E. coli gene ada has been transferred to tobacco plants by a leaf disc transformation procedure involving an Agrobacterium tumefaciens Ti plasmid. Transformed plants were shown to be transgenic for the ada message and had increased levels of O6-alkylguanine DNA alkyltransferase activity. The N-methyl-N-nitrosourea- or taurinechlorethylnitrosourea-induced inhibition of growth of calluses or of cells in suspension was considerably lower in ada-transformed than in… CONTINUE READING

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