Increased resistance to oxidative stress in transgenic plants by targeting mannitol biosynthesis to chloroplasts.

@article{Shen1997IncreasedRT,
  title={Increased resistance to oxidative stress in transgenic plants by targeting mannitol biosynthesis to chloroplasts.},
  author={Bo Shen and Richard G. Jensen and Hans J. Bohnert},
  journal={Plant physiology},
  year={1997},
  volume={113 4},
  pages={1177-83}
}
To investigate the potential role of a polyol, mannitol, in oxidative stress protection, a bacterial mannitol-1-phosphate dehydrogenase gene was targeted to chloroplasts by the addition of an amino-terminal transit peptide. Transgenic tobacco (Nicotiana tabacum) lines accumulate mannitol at concentrations ranging from 2.5 to 7 mumol/g fresh weight. Line BS1-31 accumulated approximately 100 mM mannitol in chloroplasts and was identical to the wild type in phenotype and photosynthetic performance… CONTINUE READING