Increased resistance of crosslinked cornea against enzymatic digestion

@article{Spoerl2004IncreasedRO,
  title={Increased resistance of crosslinked cornea against enzymatic digestion},
  author={Eberhard Spoerl and Gregor Wollensak and Theo Seiler},
  journal={Current Eye Research},
  year={2004},
  volume={29},
  pages={35 - 40}
}
Purpose. Collagen-crosslinking using combined riboflavin/UVA treatment has been developed by us as a new treatment for keratoconus by stiffening the collagenous matrix. Recently, we have started to use the same method for the treatment of corneal ulcers. The aim of the present study was to evaluate the influence of the crosslinking treatment on the resistance of the cornea against enzymatic degradation. Methods. 60 enucleated porcine eyes were treated with the photosensitizer riboflavin and UVA… Expand
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TLDR
Riboflavin/UVA treatment leads to a dose-dependent keratocyte damage that can be expected in human corneas down to a depth of 300 &mgr;m using a surface UVA dose of 5.4 J/cm2. Expand
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The different degree of collagen cross-linking in the corneal stroma after riboflavin/UVA treatment is reflected by the differences in the maximal shrinkage temperature of the anterior and posterior portion. Expand
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TLDR
The crosslinking effect is strongest in the anterior half of the stroma because of the rapid decrease in UVA irradiance across the corneal stroma as a result of riboflavin-enhanced UVA absorption. Expand
Corneal Resistance to Keratolysis After Collagen Crosslinking With Rose Bengal and Green Light.
TLDR
Crosslinking with RGX increased corneal resistance to digestion by collagenase comparable to that produced by riboflavin and UVA treatment, and degraded statistically significantly slower than untreated corneas. Expand
Biomechanical properties of corneal tissue after ultraviolet-A-riboflavin crosslinking.
TLDR
The collective results indicate that CXL using UVA and riboflavin enhances the biomechanical properties of the corneal tissue, which remain stable over time. Expand
Photochemical activation increases the porcine corneal stiffness and resistance to collagenase digestion.
TLDR
Collagen cross-linking by RB-PCL increased both the biomechanical stiffness and the biochemical resistance against collagenase digestion in porcine corneas, therefore to allow stabilizing and solidifier the cornea. Expand
Standard versus accelerated riboflavin–ultraviolet corneal collagen crosslinking: Resistance against enzymatic digestion
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Standard and accelerated CXL both increased corneal enzymatic resistance; however, the amount of CXL might be less when accelerated C XL is used. Expand
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Corneal collagen crosslinking induced with genipin produced a significant increase in biomechanical strength and resistance to bacterial collagenase, which could be useful in treating corneal ectasia and cornesal infectious and noninfectious diseases involving corneals melting. Expand
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References

SHOWING 1-10 OF 28 REFERENCES
Riboflavin/ultraviolet-a-induced collagen crosslinking for the treatment of keratoconus.
TLDR
Collagen crosslinking may be a new way for stopping the progression of keratectasia in patients with keratoconus and the need for penetrating keratoplasty might then be significantly reduced in keratconus. Expand
Physical crosslinking of collagen fibers: comparison of ultraviolet irradiation and dehydrothermal treatment.
TLDR
Characterization of collagen extracted from the crosslinked samples by dilute acetic acid and limited pepsin digestion indicate that both UV and DHT treatments cause fragmentation of at least a portion of the collagen molecules. Expand
Crosslinking density and resorption of dimethyl suberimidate-treated collagen.
TLDR
Collagen was purified from bovine Achilles tendon and crosslinked with dimethyl suberimidate and glutaraldehyde and GTA treatment was found to introduce more number of crosslinks than DMS, while GTA-treated collagen was highly resistant to degradation. Expand
Stress‐strain measurements of human and porcine corneas after riboflavin–ultraviolet‐A‐induced cross‐linking
TLDR
Riboflavin−UVA‐induced collagen cross‐linking led to an increase in mechanical rigidity in porcine corneas and an even greater increase in human corneal rigidity, which can be explained by the relatively larger portion of the cornea being cross‐linked in the overall thinner human cornea. Expand
Endothelial cell damage after riboflavin–ultraviolet‐A treatment in the rabbit
TLDR
In rabbit corneas with a corneal thickness less than 400 &mgr;m, the endothelial UVA dose reached a cytotoxic level of ≥0.65 J/cm2 (0.36 mW/ cm2), which is about twice the endothelium UVA doses used in the treatment of keratoconus patients. Expand
Effect of physical crosslinking methods on collagen-fiber durability in proteolytic solutions.
TLDR
The results suggest that DHT-crosslinked collagen fibers should not be used as load-bearing implants because they may retain more native structure and should exhibit greater resistance to nonspecific proteases in vivo. Expand
Synergistic effects of glucose and ultraviolet irradiation on the physical properties of collagen.
TLDR
It is strongly suggested that free-radical-dependent, glucose-derived crosslinks provide the enhanced strength and enzyme resistance observed in glucose-incorporated, UV-exposed collagen films. Expand
Collagenase Inhibitors: Rationale for Their Use in Treating Corneal Ulceration
  • M. Berman
  • Medicine
  • International ophthalmology clinics
  • 1975
TLDR
Determination of the relative efficacies of collagenase inhibitors indicates that EDTA and Ca-EDTA are about one hundred times more effective on a molar basis than L-cysteine and its derivatives, N-acetyl-L-cySteine and D-penicillamine. Expand
The specific architecture of the anterior stroma accounts for maintenance of corneal curvature.
TLDR
The rigidity of the most anterior part of the corneal stroma in extreme hydration states points to an important role in maintenance ofCorneal curvature. Expand
Cross-linking of amniotic membranes.
TLDR
Human amniotic membrane was cross-linked with chemical and radiation methods to investigate the effect of cross-linking on its physicochemical and biodegradation properties, finding no significant change was observed on the water content or protein permeation for any of the crosslinking methods and in marked contrast with cross- linking of a gelatin membrane. Expand
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