Increased ratio of targeted to random integration after transfection of chicken B cell lines.

@article{Buerstedde1991IncreasedRO,
  title={Increased ratio of targeted to random integration after transfection of chicken B cell lines.},
  author={J M Buerstedde and Shunichi Takeda},
  journal={Cell},
  year={1991},
  volume={67 1},
  pages={179-88}
}
Constructs of four different genetic loci were transfected into the avian leukosis virus-induced chicken B cell line DT40, which continues diversification of its rearranged light chain immunoglobulin gene by gene conversion. Analysis of stable transfectants revealed an unexpectedly high frequency of targeted integration into the homologous gene loci of DT40. Transcriptional activity of the target gene locus is not required, since a construct of the untranscribed ovalbumin gene also integrated… CONTINUE READING
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