Increased production of L-serine in Escherichia coli through Adaptive Laboratory Evolution.

@article{Mundhada2017IncreasedPO,
  title={Increased production of L-serine in Escherichia coli through Adaptive Laboratory Evolution.},
  author={Hemanshu Mundhada and Jose Miguel Seoane and Konstantin Schneider and Anna Koza and Hanne Bjerre Christensen and Tobias Klein and Patrick V. Phaneuf and Markus J. Herrg{\aa}rd and Adam M. Feist and Alex Toftgaard Nielsen},
  journal={Metabolic engineering},
  year={2017},
  volume={39},
  pages={
          141-150
        }
}
L-serine is a promising building block biochemical with a high theoretical production yield from glucose. Toxicity of L-serine is however prohibitive for high-titer production in E. coli. Here, E. coli lacking L-serine degradation pathways was evolved for improved tolerance by gradually increasing L-serine concentration from 3 to 100g/L using adaptive laboratory evolution (ALE). Genome sequencing of isolated clones revealed multiplication of genetic regions, as well as mutations in thrA… CONTINUE READING

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