Increased nitric oxide-dependent nitrosylation of 4,5-diaminofluorescein by oxidants: implications for the measurement of intracellular nitric oxide.

  title={Increased nitric oxide-dependent nitrosylation of 4,5-diaminofluorescein by oxidants: implications for the measurement of intracellular nitric oxide.},
  author={David Jourd’heuil},
  journal={Free radical biology \& medicine},
  volume={33 5},
  • D. Jourd’heuil
  • Published 1 September 2002
  • Chemistry, Biology
  • Free radical biology & medicine
Interfering with Nitric Oxide Measurements
The reaction products of DAF-2 with DHA and AA have been characterized using capillary electrophoresis with laser-induced fluorescence detection and electrospray mass spectrometry, indicating the importance of understanding this chemistry.
Nitrosation-modulating effect of ascorbate in a model dynamic system of coexisting nitric oxide and superoxide
Investigation of the role of ascorbate in affecting the kinetics of nitrosative chemistry in a model dynamic snystem of coexisting nitric oxide and superoxide reveals an interesting temporal distribution of superoxide, nitricoxide and peroxynitrite.
Nitric oxide (NO) detection by DAF fluorescence and chemiluminescence: a comparison using abiotic and
Because of controversies in the literature on nitric oxide (NO) production by plants, NO detection by the frequently used diaminofluorescein (DAF-2 and DAF-2DA) and by chemiluminescence were compared
Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence.
Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method.
Improved measurements of intracellular nitric oxide in intact microvessels using 4,5-diaminofluorescein diacetate.
This method overcame the dye leakage problem, enhanced the sensitivity of NO detection, and improved NO quantification, demonstrating significant advantages over existing methodologies using DAF-2.


The oxidative and nitrosative chemistry of the nitric oxide/superoxide reaction in the presence of bicarbonate.
It was concluded that NO reacts with both trans-HOONO and a CO2 adduct of ONOO- to form nitrosating species which have similar oxidation chemistry and reactivity with and NO.
Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins.
Fluorescence detection with visible light excitation and high sensitivity enabled the practical assay of NO production in living cells, and Membrane-permeable DAF-2 diacetate can be used for real-time bioimaging of NO with fine temporal and spatial resolution.
Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide.
It is proposed that superoxide dismutase may protect vascular tissue stimulated to produce superoxide and NO under pathological conditions by preventing the formation of peroxynitrite.
Distinction between Nitrosating Mechanisms within Human Cells and Aqueous Solution*
The results suggest that the functional effects of nitrosation may be organized to occur within discrete domains or compartments in biological systems when scavengers are depleted and this architecture becomes compromised.
Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore.
4,5-diaminofluorescein (DAF-2) is developed as a novel fluorescent indicator for NO that affords high sensitivity for NO and should be useful for studies of the dynamic biological actions of NO at the molecular level with fine temporal and spatial resolution.
Reaction of Superoxide and Nitric Oxide with Peroxynitrite
It is concluded that the oxidative processes initiated by the free radical intermediates formed from the decomposition of peroxynitrite are inhibited by excess production of ⋅NO or O⨪2, whereas oxidative pathways involving a direct reaction with each other are not altered.
Bicarbonate Inhibits N-Nitrosation in Oxygenated Nitric Oxide Solutions*
Bicarbonate is potentially important in determining the fate of two reactive species generated from nitric oxide, N2O3 and ONOO−, and may thus act as a regulator of NO·-induced toxicity.
Orthogonality of Calcium Concentration and Ability of 4,5-Diaminofluorescein to Detect NO*
DAF-2 can provide reliable information on NO production in biological systems regardless of the dynamic changes of Ca2+concentration, according to a recent report of divalent cation sensitivity and photoactivation of Daf-2.
Reaction kinetics for nitrosation of cysteine and glutathione in aerobic nitric oxide solutions at neutral pH. Insights into the fate and physiological effects of intermediates generated in the NO/O2 reaction.
It is demonstrated that among the primary bioorganic products of the reaction of cellular constituents with the intermediates of the NO/O2 reaction are S-nitrosothiol (S-NO) adducts, and that Chinese hamster V79 lung fibroblasts depleted of glutathione display enhanced cytotoxicity on exposure to NO.