Increased nitric oxide-dependent nitrosylation of 4,5-diaminofluorescein by oxidants: implications for the measurement of intracellular nitric oxide.

@article{Jourdheuil2002IncreasedNO,
  title={Increased nitric oxide-dependent nitrosylation of 4,5-diaminofluorescein by oxidants: implications for the measurement of intracellular nitric oxide.},
  author={David Jourd’heuil},
  journal={Free radical biology \& medicine},
  year={2002},
  volume={33 5},
  pages={
          676-84
        }
}
  • D. Jourd’heuil
  • Published 1 September 2002
  • Chemistry, Biology
  • Free radical biology & medicine
Interfering with Nitric Oxide Measurements
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Nitric oxide (NO) detection by DAF fluorescence and chemiluminescence: a comparison using abiotic and
Because of controversies in the literature on nitric oxide (NO) production by plants, NO detection by the frequently used diaminofluorescein (DAF-2 and DAF-2DA) and by chemiluminescence were compared
Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence.
Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method.
Improved measurements of intracellular nitric oxide in intact microvessels using 4,5-diaminofluorescein diacetate.
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This method overcame the dye leakage problem, enhanced the sensitivity of NO detection, and improved NO quantification, demonstrating significant advantages over existing methodologies using DAF-2.
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It was concluded that NO reacts with both trans-HOONO and a CO2 adduct of ONOO- to form nitrosating species which have similar oxidation chemistry and reactivity with and NO.
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TLDR
Fluorescence detection with visible light excitation and high sensitivity enabled the practical assay of NO production in living cells, and Membrane-permeable DAF-2 diacetate can be used for real-time bioimaging of NO with fine temporal and spatial resolution.
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The results suggest that the functional effects of nitrosation may be organized to occur within discrete domains or compartments in biological systems when scavengers are depleted and this architecture becomes compromised.
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TLDR
4,5-diaminofluorescein (DAF-2) is developed as a novel fluorescent indicator for NO that affords high sensitivity for NO and should be useful for studies of the dynamic biological actions of NO at the molecular level with fine temporal and spatial resolution.
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TLDR
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TLDR
Bicarbonate is potentially important in determining the fate of two reactive species generated from nitric oxide, N2O3 and ONOO−, and may thus act as a regulator of NO·-induced toxicity.
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TLDR
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Reaction kinetics for nitrosation of cysteine and glutathione in aerobic nitric oxide solutions at neutral pH. Insights into the fate and physiological effects of intermediates generated in the NO/O2 reaction.
TLDR
It is demonstrated that among the primary bioorganic products of the reaction of cellular constituents with the intermediates of the NO/O2 reaction are S-nitrosothiol (S-NO) adducts, and that Chinese hamster V79 lung fibroblasts depleted of glutathione display enhanced cytotoxicity on exposure to NO.
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