Increased levels of polyadenylated histone H2B mRNA accumulate during Entamoeba invadens cyst formation.

Abstract

We have isolated cDNA clones of a member of the histone H2B protein family by differential screening of an Entamoeba invadens cDNA library with cDNA probes from vegetatively growing or encysting parasites. The cDNA clones reveal two polyadenylation sites, 26 nucleotides and 31 nucleotides downstream from the stop codon. RNA species recognized by E. invadens histone H2B clones are found at increased levels during cyst formation. Histone H2B RNA could be detected in both the poly(A)+ and poly(A)- RNA fractions, with stage-specific differences in the steady state levels of the two RNAs: trophozoites contain predominantly the poly(A)- RNA, while encysting parasites express predominantly the poly(A)+ RNA. Southern blot analysis suggests that both forms are transcribed from a single copy gene.

Statistics

020406080'97'99'01'03'05'07'09'11'13'15'17
Citations per Year

114 Citations

Semantic Scholar estimates that this publication has 114 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Snchez1994IncreasedLO, title={Increased levels of polyadenylated histone H2B mRNA accumulate during Entamoeba invadens cyst formation.}, author={Lidya B. S{\'a}nchez and V. Enea and Daniel J. Eichinger}, journal={Molecular and biochemical parasitology}, year={1994}, volume={67 1}, pages={137-46} }