Increased levels of a particular phosphatidylcholine species in senescent human dermal fibroblasts in vitro

  title={Increased levels of a particular phosphatidylcholine species in senescent human dermal fibroblasts in vitro},
  author={E. Naru and Y. Takanezawa and Misako Kobayashi and Y. Misaki and K. Kaji and K. Arakane},
  journal={Human Cell},
Plasma membranes are essential components of living cells, and phospholipids are major components of cellular membranes. Here, we used liquid chromatography/mass spectrometry to investigate changes in the membrane phospholipid content that occur in association with aging. Our results indicate that the levels of a particular species of phosphatidylcholine comprised of stearic acid and arachidonic acid increased with age. To determine the reason forthe increased levels of this particular… Expand
Elucidating the metabolism of n-3 polyunsaturated fatty acids and formation of bioactive lipid mediators in human skin
Human skin has distinct lipid metabolism and production of bioactive lipid mediators that can be modulated by nutritional supplementation with omega-3 polyunsaturated fatty acids (n-3 PUFA), of whichExpand
Caveolar Vesicles in Cellular Senescence
The replicative limit of human fibroblasts has long provided a model to assess the molecular mechanisms which underlie cellular aging, and p53 mediates senescence by many other stimuli including oxidative stress, DNA damaging agents and oncogenic activation. Expand
Lipids: biomarkers of healthy aging.
The study of serum, and plasma lipidome in centenarians, and elderly individuals without age-related diseases can be a useful tool for the identification of aging biomarkers, and to understand physiological aging, and longevity. Expand
Autophagy deficient melanocytes display a senescence associated secretory phenotype that includes oxidized lipid mediators.
The secretion of inflammation related factors suggests that autophagy deficient melanocytes display a senescence associated secretory phenotype (SASP), and oxidized lipid mediators as novel components of this SASP are proposed. Expand
Human serum metabolic profiles are age dependent
The results indicate that metabolic profiles are age dependent and might reflect different aging processes, such as incomplete mitochondrial fatty acid oxidation. Expand
Metabolomics Biomarkers of Frailty in Elderly Breast Cancer Patients
These metabolomics biomarkers may give a further insight into the biochemical processes involved in the development of frailty in breast cancer patients and might be useful to refine the comprehensive geriatric assessment model. Expand
Genetic) Epidemiology of Aging
While environmental factors affecting longevity include smoking, alcohol consumption, diastolic blood pressure and C-reactive protein, APOE and FOXO3 remain the only genes consistently associated with longevity. Expand
Comparative blood and urine metabolomics analysis of healthy elderly and young male Singaporeans.
Signals in the amino acid and fatty acid metabolism pathways suggest the regulation of these pathways contributes to the aging process in Chinese Singaporeans, and further multi-omics studies including the gut microbiome and intervention studies in a larger cohort are needed. Expand
19 Caveolar Vesicles in Cellular Senescence


Polyunsaturated fatty acid metabolism of human skin fibroblasts during cellular aging.
  • D. Raederstorff, V. Loechleiter, U. Moser
  • Biology, Medicine
  • International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition
  • 1995
The results indicate that the desaturase activities decreased significantly during cellular aging of human skin fibroblasts during 30 passages. Expand
Is increased arachidonic acid release a cause or a consequence of replicative senescence?
The results indicate that some aspects of AA metabolism in normal human fibroblasts aged in culture are significantly different in comparison to early passage cells and suggest that the increasing AA release is not causal, but rather the result of in vitro aging. Expand
Modification by docosahexaenoic acid of age-induced alterations in gene expression and molecular composition of rat brain phospholipids
The results are interpreted in terms of the roles of different phospholipid molecular species in cognitive functions coupled with differential responsiveness of the genetic machinery of neurons to n-3 polyunsaturated fatty acids. Expand
Age-related changes in essential fatty acid metabolism in cultured rat heart myocytes.
Heart myocytes grew in culture for different periods of time, and a decrease in the desaturating/elongating activities for both substrates as the cells aged in culture was observed, suggesting the possibility of counteracting the effects of aging on lipid metabolism by supplementing cultures with appropriate amounts of highly unsaturated fatty acids. Expand
Effect of dietary polyunsaturated fatty acids on age-related changes in cardiac mitochondrial membranes
  • S. Pepe
  • Medicine, Biology
  • Experimental Gerontology
  • 2005
Diet rich in omega-3 PUFA reverses the age-associated membrane omega- 3:omega-6 PUFA imbalance, and dysfunctional Ca2+ metabolism, facilitating increased efficiency of mitochondrial energy production and improved tolerance of ischemia and reperfusion. Expand
Loss of functional caveolae during senescence of human fibroblasts
It is demonstrated that one of the key organizing membrane microdomains for receptor signaling, caveolae, are absent in senescence, suggesting that a fundamental loss of signal integration at the plasma membrane of senescent cells is due to the loss of signaling competent Caveolae. Expand
Phospholipid profiles in the salivary glands of rats of different ages.
The results suggest that phospholipids in the major salivary glands change with the development of rat, and the aging rats had higher total lipid contents than the young ones and had lower phospholIPid contents of the major Salivary gland. Expand
Changes in cell-surface glycosaminoglycans in human diploid fibroblasts during in vitro aging
It is concluded that the increased accumulation of heparan sulfate on the cell surface might be involved in an age-related alteration in the cell membrane. Expand
Diminished in vitro tyrosine kinase activity of the EGF receptor of senescent human fibroblasts
It is shown here that autophosphorylating activity is absent from the EGF receptor of cells which have lost their mitogenic responsiveness to EGF, and underlies a common mechanism of growth control. Expand
G protein-coupled receptor systems and their lipid environment in health disorders during aging.
The involvement of GPCRs and their lipid environment in the development of the major human pathologies associated with aging such as cancer, neurodegenerative disorders and cardiovascular pathologies is reviewed. Expand