Increased furfural tolerance due to overexpression of NADH-dependent oxidoreductase FucO in Escherichia coli strains engineered for the production of ethanol and lactate.

@article{Wang2011IncreasedFT,
  title={Increased furfural tolerance due to overexpression of NADH-dependent oxidoreductase FucO in Escherichia coli strains engineered for the production of ethanol and lactate.},
  author={Xuan Wang and Elliot Miller and Lorraine P. Yomano and Xueli Zhang and Keelnatham T Shanmugam and Lonnie O'Neal Ingram},
  journal={Applied and environmental microbiology},
  year={2011},
  volume={77 15},
  pages={5132-40}
}
Furfural is an important fermentation inhibitor in hemicellulose sugar syrups derived from woody biomass. The metabolism of furfural by NADPH-dependent oxidoreductases, such as YqhD (low K(m) for NADPH), is proposed to inhibit the growth and fermentation of xylose in Escherichia coli by competing with biosynthesis for NADPH. The discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural provided a new approach to improve furfural tolerance. Strains that produced… CONTINUE READING