Increased activity of porcine pancreatic phospholipase A2 by designed long-range electrostatic stabilisation of the transition state.

@article{Sheffield1995IncreasedAO,
  title={Increased activity of porcine pancreatic phospholipase A2 by designed long-range electrostatic stabilisation of the transition state.},
  author={Pamela Sheffield and K M Bhat and Albert J. Owen and Boyd Perry and Ian Sumner and Jim Warwicker},
  journal={Biochemical and biophysical research communications},
  year={1995},
  volume={216 3},
  pages={778-84}
}
Stabilisation of the catalytic transition state by long-range charge interactions has been tested with mutagenesis for porcine pancreatic phospholipase A2. Electrostatics calculations were used to determine locations which would interact preferentially with one part of the dipolar charge separation that is believed to develop in the transition state… CONTINUE READING