Increased SPARC accumulation during corneal repair.

  title={Increased SPARC accumulation during corneal repair.},
  author={Bridgette L Berryhill and Bradley P Kane and Brian M Stramer and Mary Elizabeth Fini and J. Hassell},
  journal={Experimental eye research},
  volume={77 1},
Keratocytes can become fibroblasts and myofibroblasts during corneal injury and wound healing. We used the in vitro bovine keratocyte repair model system, which involves culturing collagenase-isolated keratocytes in serum-free media and then adding serum or serum plus TGF-beta to the culture media to induce the fibroblast and myofibroblast phenotypes, respectively, to evaluate the synthesis of secreted products by the cells. Serum and serum plus TGF-beta rapidly induced the fibroblast… CONTINUE READING