Increased Inosine Production by a Bacillus subtilis Xanthine-Requiring Mutant Derived by Insertional Inactivation of the IMP Dehydrogenase Gene

Abstract

We have devised a direct selection for auxotrophic mutant isolation, and report here its use to obtain a xanthine-requiring mutant of B. subtilis that shows significantly improved inosine production. Insertion of a chloramphenicol acetyl transferase gene into a cloned B. subtilis IMP dehydrogenase gene on pBR322 was followed by transformation of inosine… (More)
DOI: 10.1038/nbt0889-821

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