Increased Functional Stability and Homogeneity of Viral Envelope Spikes through Directed Evolution


The functional HIV-1 envelope glycoprotein (Env) trimer, the target of anti-HIV-1 neutralizing antibodies (Abs), is innately labile and coexists with non-native forms of Env. This lability and heterogeneity in Env has been associated with its tendency to elicit non-neutralizing Abs. Here, we use directed evolution to overcome instability and heterogeneity… (More)
DOI: 10.1371/journal.ppat.1003184


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