Increased Adherence to Caco-2 Cells Caused by Disruption of the yhiE and yhiF Genes in Enterohemorrhagic Escherichia coli O157:H7

@article{Tatsuno2003IncreasedAT,
  title={Increased Adherence to Caco-2 Cells Caused by Disruption of the yhiE and yhiF Genes in Enterohemorrhagic Escherichia coli O157:H7},
  author={Ichiro Tatsuno and Keiji Nagano and Kazuki Taguchi and Li Rong and Hiroshi Mori and Chihiro Sasakawa},
  journal={Infection and Immunity},
  year={2003},
  volume={71},
  pages={2598 - 2606}
}
ABSTRACT Adherence of enterohemorrhagic Escherichia coli (EHEC) to intestinal epithelium is essential for initiation of infections, including diarrhea, and expression of the genes of the locus of enterocyte effacement (LEE) is thought to be crucial for adherence. To identify genes involved in modulating the adherent capacity, bacteria collected from an EHEC O157:H7 strain (O157Sakai) mutagenized by mini-Tn5Km2 were screened for their ability to increase the number of microcolonies (MC) on Caco… Expand
Positive effects of multiple pch genes on expression of the locus of enterocyte effacement genes and adherence of enterohaemorrhagic Escherichia coli O157 : H7 to HEp-2 cells.
TLDR
Results suggest that the pchABC genes are necessary for full virulence of EHEC O157, and demonstrate that the positive effect of pchA/B/C was caused by enhanced transcription of the LEE-encoded regulatory gene, ler. Expand
Differential Gene Expression and Adherence of Escherichia coli O157:H7 In Vitro and in Ligated Pig Intestines
TLDR
Low adherence in vitro by bacteria grown in MB was mainly due to lactose, possibly implicating the involvement of carbon catabolite repression (CCR) and/or acidic pH, and decreased expression of LEE genes and the failure of EHEC O157:H7 to adhere to epithelial cells in vitro but this repression was overcome in vivo. Expand
Coordinate Control of the Locus of Enterocyte Effacement and Enterohemolysin Genes by Multiple Common Virulence Regulators in Enterohemorrhagic Escherichia coli
TLDR
Investigations of the regulation of ehx gene expression determined that Ler also acts as an activator for Ehx expression without requiring GrlA function, indicating that transcription ofEhx is positively regulated by Ler, Grl a, and LrhA, which all act as positive regulators for LEE expression. Expand
Adherence and associated virulence gene expression in acid-treated Escherichia coli O157 : H7 in vitro and in ligated pig intestine.
TLDR
It was found that in vitro, AT O157 : H7 had significantly decreased adherence accompanied by decreased expression of stcE and toxB but not of the locus of enterocyte effacement (LEE) genes, indicating that AT and NAT O157: H7 behave differently, and that expression of their virulence genes is regulated differently in vitro from in vivo. Expand
Transient Shielding of Intimin and the Type III Secretion System of Enterohemorrhagic and Enteropathogenic Escherichia coli by a Group 4 Capsule
TLDR
It is suggested that temporal expression of the capsule, which is coordinated with that of the TTSS, is required for optimal EHEC colonization of the host intestine. Expand
Adhesion of enterohemorrhagic Escherichia coli O157:H7 to the intestinal epithelia is essential for inducing secretory IgA antibody production in the intestine of mice.
TLDR
Adhesion of EHEC to epithelial cells is essential for inducing the mucosal immune response in the intestine but not forinducing the systemic immune response. Expand
Transcription of the ehx Enterohemolysin Gene Is Positively Regulated by GrlA, a Global Regulator Encoded within the Locus of Enterocyte Effacement in Enterohemorrhagic Escherichia coli
TLDR
It is reported here that the grlR deletion significantly induced enterohemolysin (Ehx) activity of EHEC O157 on plates containing defibrinated sheep erythrocytes, suggesting that GrlA acts as a positive regulator for the ehx transcription in EH EC. Expand
Escherichia coli O157:H7 Survives within Human Macrophages: Global Gene Expression Profile and Involvement of the Shiga Toxins
TLDR
The uptake and survival of strain EDL933 during infection of human macrophages was evaluated and the initial uptake of Shiga toxins mutants was higher than that of the wild type; however, the survival rates were significantly lower at 24 h postinfection. Expand
A Positive Regulatory Loop Controls Expression of the Locus of Enterocyte Effacement-Encoded Regulators Ler and GrlA
TLDR
The results indicate that Ler and GrlA induce the expression of each other by, at least in part, counteracting the repression mediated by H-NS, revealing a novel regulatory loop controlling the optimal expression of virulence genes in A/E pathogens. Expand
Regulators Encoded in the Escherichia coli Type III Secretion System 2 Gene Cluster Influence Expression of Genes within the Locus for Enterocyte Effacement in Enterohemorrhagic E. coli O157:H7
TLDR
It is shown that mutational inhibition of two regulatory genes from the ETT2 cluster in EHEC O157:H7 leads to greatly increased secretion of proteins encoded by the LEE and to increased adhesion to human intestinal cells. Expand
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