The molecular mechanisms of DNA repair following chronic medium-dose-rate (MDR) γ-ray-induced damage remain largely unknown.
We used a cell function imager to quantitatively measure the fluorescence intensity of γ-H2A.X foci in MDR (0.015 Gy/h and 0.06 Gy/h) or high-dose-rate (HDR) (54 Gy/h) γ-ray irradiated… (More)
Figure 5. Time-course-dependent changes in the intensity of c-H2A.X foci in response to HDR (54 Gy/h) c-ray irradiation. A. The left side shows the relative ratios (IR/non-IR), at 1, 24, and 72 h after HDR (0.9 Gy/min, and total dose was 4.32 Gy) c-ray irradiations in both wild-type and scid/scid MEFs from the G1-phase fraction. The right side shows the same data using a box plot, which depicts groups of numerical data through their five-number summaries, i.e., smallest observation, lower quartile, median, upper quartile and largest observation. B. The left side shows relative ratios (IR/non-IR) at 1, 24, and 72 h after HDR (0.9 Gy/min and total dose was 4.32 Gy) c-ray irradiation in both wild-type and scid/scid MEFs from the G2/Mphase fraction. The right side shows a box plot representation of the same data. Closed circles (N) indicate wild-type MEFs, and opened circles (#) indicate scid/scid MEFs. The numbers next to the circles indicate statistical significance compared to non-irradiated cells by t-test. The numbers in the box plot indicate statistical significance between wild-type MEFs and scid/scid MEFs by t-test. Each averaged I/A was calculated from 20 replicate experiments (4 independent experiments with 5 points taken in each experiment) for detecting relative ratios. doi:10.1371/journal.pone.0045320.g005