Increase of homozygosity in centenarians revealed by a new inter-Alu PCR technique.

@article{Bonaf2001IncreaseOH,
  title={Increase of homozygosity in centenarians revealed by a new inter-Alu PCR technique.},
  author={Massimiliano Bonaf{\'e} and Maurizio Cardelli and Francesca Marchegiani and Luca Cavallone and Simona Giovagnetti and Fabiola Olivieri and Rosamaria Lisa and Carlo Pieri and Claudio Franceschi},
  journal={Experimental gerontology},
  year={2001},
  volume={36 7},
  pages={1063-73}
}
In the present study a novel inter-Alu PCR technique that allows one to detect inter-individual differences in the genomic regions flanked by Alu repetitive sequences was developed. Two primers complementary to sequences present in different Alu repeats and marked with two different fluorochromes were used in the same PCR reaction, and the PCR products were separated and analyzed by capillary electrophoresis using an automatic sequencer. The method is highly reliable, and three patterns of… CONTINUE READING