Increase in fidelity of rat liver Ile-tRNA formation by both spermine and the aminoacyl-tRNA synthetase complex.

@article{KusamaEguchi1991IncreaseIF,
  title={Increase in fidelity of rat liver Ile-tRNA formation by both spermine and the aminoacyl-tRNA synthetase complex.},
  author={K Kusama-Eguchi and M Irisawa and Shun Watanabe and Kikuko Watanabe and Kazuei Igarashi},
  journal={Archives of biochemistry and biophysics},
  year={1991},
  volume={288 2},
  pages={495-9}
}
To examine the polyamine effects on the fidelity at the aminoacylation level and the physiological significance of the existence of the aminoacyl-tRNA synthetase complex (ARSC) in animal cells, a single-chain Ile-tRNA synthetase (IRSS) was isolated from the complex by treatment with trypsin. Ile-tRNA formation by IRSS was strongly stimulated by spermine, similar to the results with ARSC. Two misacylations (Val-tRNAIle and Ile-tRNAiMet formation) by IRSS were measured. The error frequency was… CONTINUE READING

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